Clinical Evidence

Keratins Play a Significant Role in the Wound Healing Process

In vitro studies have shown that Replicine™ Bioactive Keratin increases the rates of proliferation and migration of keratinocyte cells, which play a key role in epithelialization, and that the migrating keratinocytes express increased levels of collagen IV and collagen VII, which are critical for basal membrane integrity. These basal membrane collagens join the dermal and epidermal junction to build a robust, healed skin surface.1

Applying keratin topically to the wound has also been shown in-vivo and in clinical studies to elevate the number of structural proteins made and accelerate epithelialization, thus strengthening the skin and promoting healing in recalcitrant, chronic wounds.2-7


Keratin Stimulates New Cell Growth

In an in vivo study, keratin treatment was shown to accelerate healing and closure of deep partial-thickness wounds in comparison with a conventional occlusive dressing, delivering an early onset of epithelialization stimulated by enhancing the activation of keratinocytes. Cell growth was further corroborated by gene expression analyses revealing induction of KRT6A, KRT16, and KRT17 by Day 2 postwounding.6

 

Vessel-rich dermis and interdigitation of skin layers in keratin-treated wound is indicative of stronger new skin.

Keratin dressing increases epithelial thickness of the wound edge at Day 4 versus the control, accelerating wound healing. 6

 

 By Day 5, 60% of wounds treated with liquid keratin were completely reepithelialized vs 26.7% of wounds treated with polyurethane dressings, vs 0% in the untreated group.6

 

 

 

 

Some wounds treated with keratin dressings showed complete reepithelialization after just 4 days. With liquid keratin6:

  • Statistical significance achieved at Days 5 and 6
  • 93% of wounds completely reepithelialized by Day 6


References

1.Tang L, Sierra JO, Kelly R, et al. Wool-derived keratin stimulates human keratinocyte migration and types IV and VII collagen expression. Exper Derm. 2012(21):456-476. 2. Denyer J, Marsh C, Kirsner RS. Keratin gel in the management of Epidermolysis bullosa. J Wound Care. 2015;24(10):446-450. 3. Batzer AT, Marsh C, Kirsner RS. The use of keratin-based wound products on refractory wounds. Int Wound J. 2016;13(1):110-115. 4. Davidson A, Jina NH, Marsh C, et al. Do functional keratin dressings accelerate epithelialization in human partial thickness wounds? A randomized controlled trial on skin graft donor sites. Eplasty. 2013;3:375-381. 5. Kirsner RS, Cassidy S, Marsh C, et al. Use of a keratin-based wound dressing in the management of wounds in a patient with recessive dystrophic epidermolysis bullosa. Adv Skin Wound Care. 2012;25(9):400-403. 6. Pechter PM, Gil J, Valdes J, et al. Keratin dressings speed epithelialization of deep partial-thickness wounds. Wound Rep Reg. 2012;20:236-242. 7. Than MP, Smith RA, Cassidy S, et al. Use of a keratin-based hydrogel in the management of recessive dystrophic epidermolysis bullosa. J Derm Treat. 2013;24(4):290-294.